CDA Kit ELISA

N° du produit ABIN2043263

Détail du produit CDA Kit ELISA

Antigène: CDA (Cytidine Deaminase (CDA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.28 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Cytidine Deaminase (CDA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytidine Deaminase (CDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytidine Deaminase (CDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytidine Deaminase (CDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytidine Deaminase (CDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CDA (Cytidine Deaminase (CDA))

Abstract: CDA Produits

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