IL-6 Kit ELISA

N° du produit ABIN2043681

Ce kit ELISA Rat IL-6 est un kit ELISA Colorimetric conçu pour quantifier Rat IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN2043681)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 pg/mL

Application: ELISA

Détail du produit IL-6 Kit ELISA

Seuil minimal de détection: 1.56 pg/mL

Sensibilité: < 0.48 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: High Sensitivity Interleukin 6 (IL6) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome