JAK3 Kit ELISA

N° du produit ABIN2043827

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain JAK3.

Aperçu rapide pour JAK3 Kit ELISA (ABIN2043827)

Antigène: JAK3 (Janus Kinase 3 (JAK3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit JAK3 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.066 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Janus Kinase 3 (JAK3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 3 (JAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 3 (JAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 3 (JAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Janus Kinase 3 (JAK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: JAK3 (Janus Kinase 3 (JAK3))

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response