MAPK12 Kit ELISA
Aperçu rapide pour MAPK12 Kit ELISA (ABIN2043983)
Antigène
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.072 ng/mL
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Plaque
- Pre-coated
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Protocole
- Mitogen Activated Protein Kinase 12 (MAPK12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 12 (MAPK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mitogen Activated Protein Kinase 12 (MAPK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))
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Autre désignation
- Mitogen Activated Protein Kinase 12 (MAPK12)
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Pathways
- Signalisation MAPK, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins
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