MYD88 Kit ELISA
Aperçu rapide pour MYD88 Kit ELISA (ABIN2044005)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 78.12 pg/mL
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Sensibilité
- < 28 pg/mL
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Plaque
- Pre-coated
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Protocole
- Myeloid Differentiation Factor 88 (MyD88) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Differentiation Factor 88 (MyD88). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Differentiation Factor 88 (MyD88). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Differentiation Factor 88 (MyD88), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myeloid Differentiation Factor 88 (MyD88) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
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Autre désignation
- Myeloid Differentiation Factor 88 (MyD88)
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Pathways
- Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
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