PKC epsilon Kit ELISA
Aperçu rapide pour PKC epsilon Kit ELISA (ABIN2044337)
Antigène
Voir toutes PKC epsilon (PRKCE) Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Marque
- BioAssay™
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Sensibilité
- < 0.053 ng/mL
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Protein Kinase C, epsilon (PRKCE) ELISA Kit
PRKCE Reactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivité: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivité: Souris Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivité: Humain Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivité: Souris Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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Plaque
- Pre-coated
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Protocole
- Protein Kinase C Epsilon (PKCe) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon (PKCe). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Epsilon (PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Epsilon (PKCe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase C Epsilon (PKCe) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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Autre désignation
- Protein Kinase C epsilon (PKCe)
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Pathways
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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