PDK1 Kit ELISA

N° du produit ABIN2044369

Kit ELISA Rat PDK1, test Colorimetric pour la quantification de Rat PDK1.

Aperçu rapide pour PDK1 Kit ELISA (ABIN2044369)

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit PDK1 Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Sensibilité: < 0.58 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Autre désignation: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Regulation of Carbohydrate Metabolic Process, Hepatitis C, L'effet Warburg