PDK1 Kit ELISA

N° du produit ABIN2044369

Détail du produit PDK1 Kit ELISA

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.58 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Autre désignation: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) (PDK1 Produits)

Synonymes: ATP5A Kit ELISA, B830012B01 Kit ELISA, D530020C15Rik Kit ELISA, si:zfos-979f1.3 Kit ELISA, 3-phosphoinositide dependent protein kinase-1 Kit ELISA, pyruvate dehydrogenase kinase 1 Kit ELISA, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) Kit ELISA, pyruvate dehydrogenase kinase, isoenzyme 1 Kit ELISA, pyruvate dehydrogenase kinase, isozyme 1 Kit ELISA, pyruvate dehydrogenase kinase 1 L homeolog Kit ELISA, Pdpk1 Kit ELISA, PDK1 Kit ELISA, ATP5G2 Kit ELISA, Pdk1 Kit ELISA, pdk1 Kit ELISA, pdk1.L Kit ELISA

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Regulation of Carbohydrate Metabolic Process, Hepatitis C, L'effet Warburg