KIT Ligand Kit ELISA

N° du produit ABIN2044605

Ce kit ELISA Boeuf (Vache) KIT Ligand est un kit ELISA Colorimetric conçu pour quantifier Boeuf (Vache) KIT Ligand.

Aperçu rapide pour KIT Ligand Kit ELISA (ABIN2044605)

Antigène: KIT Ligand (KITLG)

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit KIT Ligand Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 5.7 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Stem Cell Factor (SCF) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stem Cell Factor (SCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Stem Cell Factor (SCF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: Stem Cell Factor (SCF)

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway