SRD5A1 Kit ELISA

N° du produit ABIN2044607

Détail du produit SRD5A1 Kit ELISA

Antigène: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.048 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Steroid 5 Alpha Reductase 1 (SRD5a1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 1 (SRD5a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Steroid 5 Alpha Reductase 1 (SRD5a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Autre désignation: Steroid 5 alpha Reductase 1 (SRD5a1) (SRD5A1 Produits)

Synonymes: zgc:153768 Kit ELISA, 0610031P22Rik Kit ELISA, 4930435F02Rik Kit ELISA, S5AR 1 Kit ELISA, Srd5a-1 Kit ELISA, steroid 5 alpha-reductase 1 Kit ELISA, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) Kit ELISA, 3-oxo-5-alpha-steroid 4-dehydrogenase 1 Kit ELISA, SRD5A1 Kit ELISA, srd5a1 Kit ELISA, Bm1_06570 Kit ELISA, CC1G_14953 Kit ELISA, Srd5a1 Kit ELISA

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process