UPRT Kit ELISA

N° du produit ABIN2044757

Détail du produit UPRT Kit ELISA

Antigène: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.051 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Uracil Phosphoribosyltransferase (UPRT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uracil Phosphoribosyltransferase (UPRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uracil Phosphoribosyltransferase (UPRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uracil Phosphoribosyltransferase (UPRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uracil Phosphoribosyltransferase (UPRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Autre désignation: Uracil Phosphoribosyltransferase (UPRT) (UPRT Produits)

Synonymes: Gm774 Kit ELISA, FUR1 Kit ELISA, UPP Kit ELISA, uracil phosphoribosyltransferase Kit ELISA, uracil phosphoribosyltransferase homolog Kit ELISA, Uprt Kit ELISA, UPRT Kit ELISA

Pathways: Ribonucleoside Biosynthetic Process