KIT Kit ELISA
Aperçu rapide pour KIT Kit ELISA (ABIN2648535)
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Méthode de détection
Application
Type d'échantillon
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Fonction
- Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117.
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Analytical Method
- Quantitative
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Sensibilité
- 0.095 ng/ml
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Attributs du produit
- Human CD117 ELISA Assay kit is for Research Use Only.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 1 - 2 h
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Plaque
- Pre-coated
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Protocole
- The CD117 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Autre désignation
- CD117 / C-KIT
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Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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