IGFBP4 Kit ELISA
Aperçu rapide pour IGFBP4 Kit ELISA (ABIN2648687)
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Méthode de détection
Application
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Fonction
- IGFBP-4 ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) in biological fluids.
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Analytical Method
- Quantitative
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Sensibilité
- 0.625 ng/ml
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Attributs du produit
- The IGFBP-4 ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 50 μL
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Durée du test
- 2 h
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Plaque
- Pre-coated
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Protocole
- The biotinylated antibody binds to the streptavidin coated microtiter plate. The IGFBP-4 of the standards/samples binds to this antibody. The biotinylated antibody and the standards/ samples are incubated on the microtiter plate at the same time. After a washing step the bound IGFBP-4, is then detected by a horseradish peroxidase-conjugated anti-IGFBP-4 antibody. After a second washing step the conjugate is added and incubated. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of IGFBP-4.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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Autre désignation
- IGFBP-4
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Pathways
- Signalisation WNT, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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