IL-3 Kit ELISA

N° du produit ABIN2648694

Ce kit ELISA Humain IL-3 est un kit ELISA Colorimetric conçu pour quantifier Humain IL-3.

Aperçu rapide pour IL-3 Kit ELISA (ABIN2648694)

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit IL-3 Kit ELISA

Fonction: Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensibilité: 15 pg/ml

Attributs du produit: Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole: Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL-3

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy