IL-1 beta Kit ELISA

N° du produit ABIN2648758

Kit ELISA Souris IL-1 beta, test Colorimetric pour la quantification de Souris IL-1 beta.

Aperçu rapide pour IL-1 beta Kit ELISA (ABIN2648758)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Souris

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit IL-1 beta Kit ELISA

Fonction: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse γ-interferon (IL-1β) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensibilité: 7 pg/ml

Attributs du produit: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 1 Beta (IL-1β) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 1 Beta (IL-1β) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 1 Beta (IL-1β) is added to the wells and binds to the combination of capture antibody-IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 1 Beta (IL-1β) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 1 Beta (IL-1β) standard dilutions and Interleukin 1 Beta (IL-1β) sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL-1beta

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome