2-ThioBarbituric Acid Reactive Substances (TBARS) Kit ELISA

N° du produit ABIN2648832

Détail du produit

Antigène: 2-ThioBarbituric Acid Reactive Substances (TBARS)

Reactivité: Chemical

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Urine

Sensibilité: 0.5 μM

Attributs du produit: 2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 0.5 h

Plaque: Pre-coated

Protocole: This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: 2-ThioBarbituric Acid Reactive Substances (TBARS)