Thyroglobulin Kit ELISA

N° du produit ABIN2648834

Détail du produit Thyroglobulin Kit ELISA

Antigène: Thyroglobulin (TG)

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum

Specificité: Ultrasensitive ELISA

Sensibilité: 0.03 ng/mL

Attributs du produit: Biochemically, Tg is to be understood as a rather complex family of molecules. It is micro heterogeneous with inter- and intra-individual variations (iodination degree, carbohydrate contents etc.). Dimers and several fragments also exist. Additional heterogeneity is due to malignant de-differentiation. Specifically and unspecifically interfering factors in individual sera cause further problems. Therefore, the Tg determination still represents a rather ambitious method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 25 μL

Plaque: Pre-coated

Protocole: Tg of serum samples, controls and calibrators react with the solid phase bound anti-hTg antibody during the first incubation period of 2.5 hours at room temperature. After removal of the non-bound components by a washing step anti-hTg HRP conjugate interacts with the Tg molecules trapped by the immobilized anti-hTg antibody during the second incubation at room temperature over night (> 15 hours). The sandwich type com-plexes formed are separated from unbound material by a further washing step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min, turning the solution from blue to yellow.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Thyroglobulin (TG)

Autre désignation: Tg (Thyroglobulin) (TG Produits)

Synonymes: TG Kit ELISA, cb717 Kit ELISA, AITD3 Kit ELISA, TGN Kit ELISA, Tgn Kit ELISA, cog Kit ELISA, thyroglobulin Kit ELISA, TG Kit ELISA, tg Kit ELISA, LOC701526 Kit ELISA, LOC701756 Kit ELISA, LOC100434297 Kit ELISA, Tg Kit ELISA

Pathways: Thyroid Hormone Synthesis