IL12 Kit ELISA

N° du produit ABIN2690562

Ce kit ELISA Humain IL12 est un kit ELISA Colorimetric conçu pour quantifier Humain IL12. Ce Kit ELISA a été cité dans 2+ publications.

Aperçu rapide pour IL12 Kit ELISA (ABIN2690562)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Supernatant

Détail du produit IL12 Kit ELISA

Fonction: The Human IL-12 (p40) ELISA Kit II is for the quantitative determination of human IL-12 (p40) in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Information d'application

Indications d'application: The ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance "Recovery" and "Linearity" sections.

Commentaires:

BD OptEIA™ Human IL-12 (p40) ELISA Kit II

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

Références pour IL12 Kit ELISA (ABIN2690562)

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." dans: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12

Sujet: Interleukin 12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35)and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMCs producea manyfold excess of IL-12 p40 monomer over the bioactive p70 heterodimer.The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20x less effectiveness than the IL-12 p70 homodimer.

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome