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LGALS8 Kit ELISA
LGALS8
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.16-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703042
Détail du produit LGALS8 Kit ELISA
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Antigène
Voir toutes LGALS8 Kits ELISA
LGALS8
(Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))
Reactivité
Toutes les réactivités sur LGALS8 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.16-40 ng/mL
Seuil minimal de détection
0.16 ng/mL
Application
ELISA
Fonction
Human Galectin-8 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Human Galectin-8 (LGALS8)
Sensibilité
0.16 ng/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes LGALS8 Kits ELISA
LGALS8
(Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))
Autre désignation
Galectin-8 (LGALS8 Produits )
Synonymes
LGALS8 Kit ELISA, 1200015E08Rik Kit ELISA, AI326142 Kit ELISA, D13Ertd524e Kit ELISA, Lgals-8 Kit ELISA, galectin-8 Kit ELISA, Gal-8 Kit ELISA, PCTA-1 Kit ELISA, PCTA1 Kit ELISA, Po66-CBP Kit ELISA, xgalectin-VIIIa Kit ELISA, galectin 8 Kit ELISA, lectin, galactose binding, soluble 8 Kit ELISA, lectin, galactoside binding soluble 8 S homeolog Kit ELISA, LGALS8 Kit ELISA, Lgals8 Kit ELISA, lgals8.S Kit ELISA
Sujet
Gene Names: LGALS8 Protein names: Galectin-8 (Gal-8) (Po66 carbohydrate-binding protein) (Po66-CBP) (Prostate carcinoma tumor antigen 1) (PCTA-1)
ID gène
3964
UniProt
O00214
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