GPC1 Kit ELISA

N° du produit ABIN2703059

Détail du produit GPC1 Kit ELISA

Antigène: GPC1 (Glypican 1 (GPC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-40 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Fonction: Human Glypican 1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Glypican-1 (GPC1)

Sensibilité: 0.15 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples3 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour GPC1 Kit ELISA (ABIN2703059)

Monteforte, Lam, Sherman, Henderson, Sligar, Spencer, Tang, Dunn, Baker: "* Glioblastoma Exosomes for Therapeutic Angiogenesis in Peripheral Ischemia." dans: Tissue engineering. Part A, Vol. 23, Issue 21-22, pp. 1251-1261, (2018) (PubMed).

Détail du antigène

Antigène: GPC1 (Glypican 1 (GPC1))

Autre désignation: Glypican 1 (GPC1 Produits)

Synonymes: gpc1 Kit ELISA, zgc:63947 Kit ELISA, GPC1 Kit ELISA, Glypican-1 Kit ELISA, glypican Kit ELISA, AI462976 Kit ELISA, HSPG M12 Kit ELISA, HSPGM12 Kit ELISA, glypican 1b Kit ELISA, glypican 1 Kit ELISA, gpc1b Kit ELISA, GPC1 Kit ELISA, gpc1 Kit ELISA, Gpc1 Kit ELISA

Sujet: Gene Names: GPC1
Protein names: Glypican-1 [Cleaved into: Secreted glypican-1]

ID gène: 2817

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation