IL12 Kit ELISA

N° du produit ABIN2703167

Kit ELISA Souris IL12, test Colorimetric pour la quantification de Souris IL12 et a été mentionné dans 8+ publications.

Aperçu rapide pour IL12 Kit ELISA (ABIN2703167)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 5-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit IL12 Kit ELISA

Seuil minimal de détection: 5 pg/mL

Fonction: Mouse IL-12 p40/p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Sensibilité: 5 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples10 - 100 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour IL12 Kit ELISA (ABIN2703167)

Shanmugam, Wang, Markand, Perry, Tawfik, Zorrilla, Ganapathy, Smith: "Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." dans: Journal of neurochemistry, (2015) (PubMed).

Spreadbury, Ochoa-Cortes, Ibeakanma, Martin, Hurlbut, Vanner: "Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling." dans: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 27, Issue 3, pp. 347-55, (2015) (PubMed).

Ghosh, Mukherjee, Choudhury, Gupta, Adhikary, Baral, Chattopadhyay: "Reactive oxygen species in the tumor niche triggers altered activation of macrophages and immunosuppression: Role of fluoxetine." dans: Cellular signalling, Vol. 27, Issue 7, pp. 1398-412, (2015) (PubMed).

Bishayi, Bandyopadhyay, Majhi, Adhikary: "Effect of exogenous MCP-1 on TLR-2 neutralized murine macrophages and possible mechanisms of CCR-2/TLR-2 and MCP-1 signalling during Staphylococcus aureus infection." dans: Immunobiology, Vol. 220, Issue 3, pp. 350-62, (2015) (PubMed).

Abo Elnazar, Ghazy, Ghoneim, Taha, Abouelella: "Effect of ultra violet irradiation on the interplay between Th1 and Th2 lymphocytes." dans: Frontiers in pharmacology, Vol. 6, pp. 56, (2015) (PubMed).

Ghazy, Abu El-Nazar, Ghoneim, Taha, Abouelella: "Effect of murine exposure to gamma rays on the interplay between Th1 and Th2 lymphocytes." dans: Frontiers in pharmacology, Vol. 6, pp. 74, (2015) (PubMed).

Fulzele, Krause, Panaroni, Saini, Barry, Liu, Lotinun, Baron, Bonewald, Feng, Chen, Weinstein, Wu, Kronenberg, Scadden, Divieti Pajevic: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." dans: Blood, Vol. 121, Issue 6, pp. 930-9, (2013) (PubMed).

Matsushita, Seike, Okawa, Kadawaki, Ohtsu: "Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis." dans: Experimental dermatology, Vol. 21, Issue 9, pp. 714-5, (2012) (PubMed).

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 p40/70

Sujet: Gene Names: Il12b
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)

ID gène: 16160

UniProt: P43432

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome