IL12 Kit ELISA

N° du produit ABIN2703170

Détail du produit IL12 Kit ELISA

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-10000 pg/mL

Seuil minimal de détection: 40 pg/mL

Application: ELISA

Fonction: Rhesus Monkey IL-12 p70 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects Rhesus Monkey IL-12 p70 and human IL-12 p70. Other species not determined.

Sensibilité: 40 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12p70 (IL12 Produits)

Synonymes: CLMF Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12 Kit ELISA, LOC100217488 Kit ELISA, IL-12p35 Kit ELISA, Il-12a Kit ELISA, Ll12a Kit ELISA, p35 Kit ELISA, IL12p40 Kit ELISA, IL12B Kit ELISA, IL-12 p35 Kit ELISA, interleukin 12B Kit ELISA, interleukin 12A Kit ELISA, interleukin 12b Kit ELISA, interleukin 12a Kit ELISA, Interleukin 12 level Kit ELISA, IL12B Kit ELISA, IL12A Kit ELISA, Il12b Kit ELISA, Il12a Kit ELISA, IL12 Kit ELISA, il12a Kit ELISA

Sujet: Gene Names: IL12A
Protein names: Interleukin-12 subunit alpha (IL-12A) (Cytotoxic lymphocyte maturation factor 35 kDa subunit) (CLMF p35) (IL-12 subunit p35)

ID gène: 703205

UniProt: P48091

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome