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TFPI Kit ELISA
TFPI
Reactivité: Humain
Colorimetric
Sandwich ELISA
5-1800 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703483
Détail du produit TFPI Kit ELISA
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Antigène
Voir toutes TFPI Kits ELISA
TFPI
(Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
Reactivité
Toutes les réactivités sur TFPI Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
5-1800 pg/mL
Seuil minimal de détection
5 pg/mL
Application
ELISA
Fonction
Human TFPI ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair detects human TFPI. Other species not determined.
Sensibilité
5 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples50 - 500 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TFPI Kits ELISA
TFPI
(Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
Autre désignation
TFPI (TFPI Produits )
Synonymes
EPI Kit ELISA, LACI Kit ELISA, TFI Kit ELISA, TFPI1 Kit ELISA, A630013F22Rik Kit ELISA, AW552122 Kit ELISA, TFPI Kit ELISA, si:dz44f8.1 Kit ELISA, tfpib Kit ELISA, wu:fb92g01 Kit ELISA, zgc:152844 Kit ELISA, zgc:158592 Kit ELISA, tissue factor pathway inhibitor Kit ELISA, tissue factor pathway inhibitor a Kit ELISA, TFPI Kit ELISA, Tfpi Kit ELISA, tfpia Kit ELISA
Sujet
Gene Names: TFPI LACI TFPI1 Protein names: Tissue factor pathway inhibitor (TFPI) (Extrinsic pathway inhibitor) (EPI) (Lipoprotein-associated coagulation inhibitor) (LACI)
ID gène
7035
UniProt
P10646
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