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TGFBR3 Kit ELISA
TGFBR3
Reactivité: Humain
Colorimetric
Sandwich ELISA
40-10.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703487
Détail du produit TGFBR3 Kit ELISA
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Antigène
Voir toutes TGFBR3 Kits ELISA
TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Reactivité
Toutes les réactivités sur TGFBR3 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
40-10.000 pg/mL
Seuil minimal de détection
40 pg/mL
Application
ELISA
Fonction
Human TGF beta R3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair shows no cross-reactivity with recombinant human (rh) TGF-beta RI, RII, RIIb, TGF-beta 1, beta 2, beta 3, recombinant mouse TGF-beta RIII.
Sensibilité
40 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples20 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TGFBR3 Kits ELISA
TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Autre désignation
TGF-beta R3 (TGFBR3 Produits )
Synonymes
TGFBR3 Kit ELISA, DKFZp469I0915 Kit ELISA, BGCAN Kit ELISA, betaglycan Kit ELISA, 1110036H20Rik Kit ELISA, AU015626 Kit ELISA, AW215636 Kit ELISA, TBRIII Kit ELISA, transforming growth factor beta receptor 3 Kit ELISA, transforming growth factor, beta receptor III Kit ELISA, TGFBR3 Kit ELISA, tgfbr3 Kit ELISA, Tgfbr3 Kit ELISA
Sujet
Gene Names: TGFBR3 Protein names: Transforming growth factor beta receptor type 3 (TGF-beta receptor type 3) (TGFR-3) (Betaglycan) (Transforming growth factor beta receptor III) (TGF-beta receptor type III)
ID gène
7049
UniProt
Q03167
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