TGFBR2 Kit ELISA

N° du produit ABIN2703488

Détail du produit TGFBR2 Kit ELISA

Antigène: TGFBR2 (Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.5-500 pg/mL

Seuil minimal de détection: 1.5 pg/mL

Application: ELISA

Fonction: Human TGF beta R2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human TGF-beta R2. Other species not determined yet.

Sensibilité: 15 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples20 - 200 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TGFBR2 (Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))

Autre désignation: TGF beta R2 (TGFBR2 Produits)

Synonymes: AAT3 Kit ELISA, FAA3 Kit ELISA, LDS1B Kit ELISA, LDS2B Kit ELISA, MFS2 Kit ELISA, RIIC Kit ELISA, TAAD2 Kit ELISA, TGFR-2 Kit ELISA, TGFbeta-RII Kit ELISA, 1110020H15Rik Kit ELISA, AU042018 Kit ELISA, DNIIR Kit ELISA, RIIDN Kit ELISA, TBR-II Kit ELISA, TbetaR-II Kit ELISA, TbetaRII Kit ELISA, TGFBR2 Kit ELISA, TBETA-RII Kit ELISA, TGFBRII Kit ELISA, TGF-beta 2 Kit ELISA, Tgfbr2T Kit ELISA, cb537 Kit ELISA, tgfbr2 Kit ELISA, wu:fj05c10 Kit ELISA, zgc:110498 Kit ELISA, transforming growth factor beta receptor 2 Kit ELISA, transforming growth factor, beta receptor II Kit ELISA, transforming growth factor, beta receptor 2 Kit ELISA, transforming growth factor beta receptor 2b Kit ELISA, TGFBR2 Kit ELISA, Tgfbr2 Kit ELISA, tgfbr2b Kit ELISA

Sujet: Gene Names: TGFBR2
Protein names: TGF-beta receptor type-2 (TGFR-2) (EC 2.7.11.30) (TGF-beta type II receptor) (Transforming growth factor-beta receptor type II) (TGF-beta receptor type II) (TbetaR-II)

ID gène: 7048

UniProt: P37173