Uromodulin Kit ELISA
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- Antigène Voir toutes Uromodulin (UMOD) Kits ELISA
- Uromodulin (UMOD)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 40-10.000 pg/mL
- Seuil minimal de détection
- 40 pg/mL
- Application
- ELISA
- Fonction
- Human Uromodulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair detects human Uromodulin. Other species not determined.
- Sensibilité
- 40 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples200 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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-
Urinary biomarkers of chronic allograft nephropathy." dans: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).
: "
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Urinary biomarkers of chronic allograft nephropathy." dans: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).
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- Antigène Voir toutes Uromodulin (UMOD) Kits ELISA
- Uromodulin (UMOD)
- Autre désignation
- Uromodulin (UMOD Produits)
- Synonymes
- ADMCKD2 Kit ELISA, FJHN Kit ELISA, HNFJ Kit ELISA, HNFJ1 Kit ELISA, MCKD2 Kit ELISA, THGP Kit ELISA, THP Kit ELISA, urehr4 Kit ELISA, uromodulin Kit ELISA, UMOD Kit ELISA, Umod Kit ELISA
- Sujet
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Gene Names: UMOD
Protein names: Uromodulin (Tamm-Horsfall urinary glycoprotein) (THP) [Cleaved into: Uromodulin, secreted form]
- ID gène
- 7369
- UniProt
- P07911
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