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ZAP70 Kit ELISA

ZAP70 Reactivité: Humain pTyr493 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2747919
  • Antigène Voir toutes ZAP70 Kits ELISA
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Épitope
    pTyr493
    Reactivité
    • 9
    • 5
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-ZAP70 (Y493) ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes ZAP70 Kits ELISA
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Autre désignation
    ZAP70 (ZAP70 Produits)
    Synonymes
    SRK Kit ELISA, STCD Kit ELISA, STD Kit ELISA, TZK Kit ELISA, ZAP-70 Kit ELISA, 70kDa Kit ELISA, AI327364 Kit ELISA, Srk Kit ELISA, mrtle Kit ELISA, mur Kit ELISA, zap70 Kit ELISA, MGC80670 Kit ELISA, MGC88962 Kit ELISA, ZAP70 Kit ELISA, zgc:110383 Kit ELISA, zeta chain of T-cell receptor associated protein kinase 70 Kit ELISA, putative tyrosine-protein kinase zap-70 Kit ELISA, zeta-chain (TCR) associated protein kinase Kit ELISA, zeta chain of T cell receptor associated protein kinase 70 Kit ELISA, zeta-chain (TCR) associated protein kinase 70kDa Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa Kit ELISA, tyrosine-protein kinase ZAP-70 Kit ELISA, ZAP70 Kit ELISA, Smp_149470 Kit ELISA, Zap70 Kit ELISA, zap70.L Kit ELISA, zap70 Kit ELISA, LOC707555 Kit ELISA, LOC100342021 Kit ELISA, LOC100390922 Kit ELISA, LOC100542877 Kit ELISA
    Sujet
    Zeta-Chain Associated Protein Kinase 70 (ZAP70) phosphorylated at Tyrosine-493
    ID gène
    22637
    UniProt
    P43403
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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