ZAP70 Kit ELISA

N° du produit ABIN4885072

Détail du produit ZAP70 Kit ELISA

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human ZAP70 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70 (ZAP70 Produits)

Synonymes: SRK Kit ELISA, STCD Kit ELISA, STD Kit ELISA, TZK Kit ELISA, ZAP-70 Kit ELISA, 70kDa Kit ELISA, AI327364 Kit ELISA, Srk Kit ELISA, mrtle Kit ELISA, mur Kit ELISA, zap70 Kit ELISA, MGC80670 Kit ELISA, MGC88962 Kit ELISA, ZAP70 Kit ELISA, zgc:110383 Kit ELISA, zeta chain of T-cell receptor associated protein kinase 70 Kit ELISA, putative tyrosine-protein kinase zap-70 Kit ELISA, zeta-chain (TCR) associated protein kinase Kit ELISA, zeta chain of T cell receptor associated protein kinase 70 Kit ELISA, zeta-chain (TCR) associated protein kinase 70kDa Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa Kit ELISA, tyrosine-protein kinase ZAP-70 Kit ELISA, ZAP70 Kit ELISA, Smp_149470 Kit ELISA, Zap70 Kit ELISA, zap70.L Kit ELISA, zap70 Kit ELISA, LOC707555 Kit ELISA, LOC100342021 Kit ELISA, LOC100390922 Kit ELISA, LOC100542877 Kit ELISA

ID gène: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway