IL-8 Kit ELISA

N° du produit ABIN2748270

Détail du produit IL-8 Kit ELISA

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Macaque rhésus

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.4-300 pg/mL

Seuil minimal de détection: 0.4 pg/mL

Application: ELISA

Fonction: Rhesus Monkey IL-8 (CXCL8) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Rhesus Macaque Interleukin 8 (CXCL8)

Sensibilité: 0.4 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples5 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: IL-8 (IL8 Produits)

Synonymes: CXCL8 Kit ELISA, GCP-1 Kit ELISA, GCP1 Kit ELISA, LECT Kit ELISA, LUCT Kit ELISA, LYNAP Kit ELISA, MDNCF Kit ELISA, MONAP Kit ELISA, NAF Kit ELISA, NAP-1 Kit ELISA, NAP1 Kit ELISA, IL-8 Kit ELISA, AMCF-I Kit ELISA, il8 Kit ELISA, IL8 Kit ELISA, si:dkey-151b16.2 Kit ELISA, cxcli2 Kit ELISA, cxcl8 Kit ELISA, gcp-1 Kit ELISA, il-8 Kit ELISA, lynap Kit ELISA, mdncf Kit ELISA, monap Kit ELISA, nap-1 Kit ELISA, C-X-C motif chemokine ligand 8 Kit ELISA, interleukin 8 Kit ELISA, chemokine (C-X-C motif) ligand 8a Kit ELISA, chemokine (C-X-C motif) ligand 1-like Kit ELISA, chemokine (C-X-C motif) ligand 8 L homeolog Kit ELISA, CXCL8 Kit ELISA, il8 Kit ELISA, cxcl8a Kit ELISA, IL8 Kit ELISA, Cxcl8 Kit ELISA, LOC422654 Kit ELISA, cxcl8.L Kit ELISA

Sujet: Gene Names: CXCL8 IL8
Protein names: Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8)

ID gène: 613028

UniProt: P67813

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy