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LCK Kit ELISA

LCK Reactivité: Humain pTyr394 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748305
  • Antigène Voir toutes LCK Kits ELISA
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Épitope
    pTyr394, total
    Reactivité
    • 6
    • 4
    • 4
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-LCK (Y394) and Total LCK ELISA Kit. This assay semi-quantitatively measures phophorylated LCK (Tyr394) and Total LCK in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human LCK phosphorylated at Tyrosine-394 and total LCK.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product LCK Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.Add 100 μL of sample or positive control to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared primary antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared 1X HRP-Streptavidin to each well.Incubate 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes LCK Kits ELISA
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Autre désignation
    LCK (LCK Produits)
    Synonymes
    zgc:136695 Kit ELISA, LCK Kit ELISA, Hck-3 Kit ELISA, Lsk Kit ELISA, Lskt Kit ELISA, p56 Kit ELISA, p56Lck Kit ELISA, LSK Kit ELISA, YT16 Kit ELISA, p56lck Kit ELISA, pp58lck Kit ELISA, P56LCK Kit ELISA, tkl Kit ELISA, Lck1 Kit ELISA, Lcktkr Kit ELISA, LCK proto-oncogene, Src family tyrosine kinase Kit ELISA, lymphocyte protein tyrosine kinase Kit ELISA, lck Kit ELISA, LCK Kit ELISA, Lck Kit ELISA
    Sujet
    Lymphocyte-specific Protein Tyrosine Kinase (LCK)
    ID gène
    3932
    UniProt
    P06239
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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