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Reticulon 4 Kit ELISA

RTN4 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN2748436
  • Antigène Voir toutes Reticulon 4 (RTN4) Kits ELISA
    Reticulon 4 (RTN4)
    Reactivité
    • 4
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat Nogo-A (Reticulon 4A) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Rat Nogo-A. Other species not yet determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Reticulon 4 (RTN4) Kits ELISA
    Reticulon 4 (RTN4)
    Autre désignation
    Nogo-A (RTN4 Produits)
    Synonymes
    ASY Kit ELISA, NI220/250 Kit ELISA, NOGO Kit ELISA, NOGO-A Kit ELISA, NOGOC Kit ELISA, NSP Kit ELISA, NSP-CL Kit ELISA, Nbla00271 Kit ELISA, Nbla10545 Kit ELISA, Nogo-B Kit ELISA, Nogo-C Kit ELISA, RTN-X Kit ELISA, RTN4-A Kit ELISA, RTN4-B1 Kit ELISA, RTN4-B2 Kit ELISA, RTN4-C Kit ELISA, RTN4-Cw Kit ELISA, DKFZp459C0314 Kit ELISA, 1110020G17Rik Kit ELISA, AA407876 Kit ELISA, AA409940 Kit ELISA, AA960376 Kit ELISA, C130026I10Rik Kit ELISA, NgA Kit ELISA, Nogo-A Kit ELISA, mKIAA0886 Kit ELISA, mKIAA4153 Kit ELISA, NI-250 Kit ELISA, Nogo Kit ELISA, Vp20 Kit ELISA, r Kit ELISA, rat N Kit ELISA, rat NogoA Kit ELISA, reticulon-4 Kit ELISA, nogo Kit ELISA, rtn4 Kit ELISA, wu:fb17a02 Kit ELISA, wu:fb22f12 Kit ELISA, wu:fd61a07 Kit ELISA, zgc:92163 Kit ELISA, reticulon 4 Kit ELISA, reticulon 4a Kit ELISA, RTN4 Kit ELISA, rtn4 Kit ELISA, Rtn4 Kit ELISA, rtn4a Kit ELISA
    Sujet
    Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein)
    ID gène
    83765
    UniProt
    Q9JK11
    Pathways
    Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome
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