ERBB3 Kit ELISA

N° du produit ABIN2859250

Détail du produit ERBB3 Kit ELISA

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Épitope: AA 20-643

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ERBB3/Her3

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: NSO, S20-T643

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S20-T643

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Epithelial tissues and brain.

Plaque: Pre-coated

Protocole: human ERBB3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ERBB3 has been precoated onto 96-well plates. Standards (NSO, S20-T643) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ERBB3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ERBB3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ERBB3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ERBB3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.65, Standard deviation: 0.086, CV(%): 5.2
• Sample 2: n=16, Mean(ng/ml): 4.4, Standard deviation: 0.198, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 6.25, Standard deviation: 0.294, CV(%): 4.7,
• Sample 1: n=24, Mean(ng/ml): 1.37, Standard deviation: 0.081, CV(%): 5.9
• Sample 2: n=24, Mean(ng/ml): 3.56, Standard deviation: 0.182, CV(%): 5.1
• Sample 3: n=24, Mean(ng/ml): 7.19, Standard deviation: 0.446, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Autre désignation: ERBB3 (ERBB3 Produits)

Synonymes: ErbB-3 Kit ELISA, HER3 Kit ELISA, LCCS2 Kit ELISA, MDA-BF-1 Kit ELISA, c-erbB-3 Kit ELISA, c-erbB3 Kit ELISA, erbB3-S Kit ELISA, p180-ErbB3 Kit ELISA, p45-sErbB3 Kit ELISA, p85-sErbB3 Kit ELISA, ERBB3 Kit ELISA, C76256 Kit ELISA, Erbb-3 Kit ELISA, Erbb3r Kit ELISA, Her3 Kit ELISA, nuc-ErbB3 Kit ELISA, erb-b2 receptor tyrosine kinase 3 Kit ELISA, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) Kit ELISA, ERBB3 Kit ELISA, Erbb3 Kit ELISA

Sujet:

Protein Function: Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. .

Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

Synonyms: Receptor tyrosine-protein kinase erbB-3,2.7.10.1,Proto-oncogene-like protein c-ErbB-3,Tyrosine kinase-type cell surface receptor HER3,ERBB3,HER3,

Full Gene Name: Receptor tyrosine-protein kinase erbB-3

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

ID gène: 2065

UniProt: P21860

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway