MAPK14 Kit ELISA

N° du produit ABIN2950156

Ce kit ELISA Souris MAPK14 est un kit ELISA Colorimetric conçu pour quantifier Souris MAPK14.

Aperçu rapide pour MAPK14 Kit ELISA (ABIN2950156)

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.25 ng/mL - 80 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit MAPK14 Kit ELISA

Seuil minimal de détection: 1.25 ng/mL

Fonction: Mouse Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA Kit

Analytical Method: Quantitative

Sensibilité: 0.75 ng/mL

Ingrédients: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Matériel non inclus:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Information d'application

Indications d'application: Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plaque: Pre-coated

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of MAPK14 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of MAPK14 were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Date de péremption: 6 months

Détail du antigène

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Autre désignation: Mitogen-Activated Protein Kinase 14

Sujet:

Ensembl: ENSMUST00000004990 [P47811-3], ENSMUST00000062694 [P47811-1], ENSMUST00000114752 [P47811-4], ENSMUST00000114754 [P47811-4], ENSMUST00000114758 [P47811-2]

UniProt Entry Name: MK14_MOUSE

ID gène: 26416

NCBI Accession: NP_036081

UniProt: P47811

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins