PDK4 Kit ELISA

N° du produit ABIN2950987

Ce kit ELISA Porc PDK4 est un kit ELISA Colorimetric conçu pour quantifier Porc PDK4.

Aperçu rapide pour PDK4 Kit ELISA (ABIN2950987)

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit PDK4 Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Pig Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit

Analytical Method: Quantitative

Sensibilité: 0.94 ng/mL

Ingrédients: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Matériel non inclus:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Information d'application

Indications d'application: Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plaque: Pre-coated

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of PDK4 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of PDK4 were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Date de péremption: 6 months

Détail du antigène

Antigène: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Autre désignation: Pyruvate Dehydrogenase Kinase Isozyme 4

UniProt: C1IHT9

Pathways: Signalisation PI3K-Akt, Signalisation Hedgehog, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg