HSP90 Kit ELISA

N° du produit ABIN2964826

Détail du produit HSP90 Kit ELISA

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivité: Humain, Chévre, Zebra Finch

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.44 ng/mL - 28 ng/mL

Seuil minimal de détection: 0.44 ng/mL

Application: ELISA

Fonction: Colorimetric detection of HSP90 alpha

Type d'échantillon: Blood, Cell Lysate, Serum, Tissue Samples

Analytical Method: Quantitative

Sensibilité: 0.117 ng/mL

Attributs du produit: ELISA kit used to quantitate HSP90 alpha concentration in samples.

Ingrédients:

• Anti-Hsp90a Immunoassay Plate
• 5X Hsp90a Extraction Reagent
• Recombinant Hsp90a Standard
• Standard and Sample Diluent
• 10X Wash Buffer Concentrate
• Anti-Hsp90a Biotinylated Antibody Concentrate
• Anti-Hsp90a Biotinylated Antibody Diluent
• Streptavidin: HRP Concentrate
• Streptavidin: HRP Diluent
• TMB Substrate
• Stop Solution

Matériel non inclus: - Ultra pure water
- Additional reagents and materials for cell lysate and tissue extract preparation, including protease inhibitors
- Precision pipettors, with disposable plastic tips
- Polypropylene or polyethylene tubes to prepare samples − do not use polystyrene, polycarbonate or glass tubes
- A container to prepare 1X Wash Buffer
- A wash bottle or an automated 96-well plate washer

Information d'application

Durée du test: 0.5 h

Plaque: Pre-coated

Protocole:

1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 100 μL of Standard or sample to appropriate wells.
3. Cover plate with Plate Sealer and incubate at 37 °C for 1 hour.
4. Wash plate four times with 1X Wash Buffer.
5. Add 100 μL of Biotinylated Antibody Working Solution to each well.
6. Cover plate with Plate Sealer and incubate at 37 °C for 1 hour.
7. Wash plate four times with 1X Wash Buffer.
8. Add 100 μL of Streptavidin-HRP Working Solution to each well.
9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
10. Wash plate four times with 1X Wash Buffer.
11. Add 100 μL of TMB Substrate to each well.
12. Develop the plate in the dark at room temperature for 30 minutes.
13. Stop reaction by adding 100 μL of Stop Solution to each well.
14. Measure absorbance on a plate reader at 450 nm.

Procédure de l'essai:

1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 100 μL of Standard or sample to appropriate wells.
3. Cover plate with Plate Sealer and incubate at 37 °C for 1 hour.
4. Wash plate four times with 1X Wash Buffer.
5. Add 100 μL of Biotinylated Antibody Working Solution to each well.
6. Cover plate with Plate Sealer and incubate at room temperature, 20-25 °C for 1 hour.
7. Wash plate four times with 1X Wash Buffer.
8. Add 100 μL of Streptavidin-HRP Working Solution to each well.
9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
10. Wash plate four times with 1X Wash Buffer.
11. Add 100 μL of TMB Substrate to each well.
12. Develop the plate in the dark at room temperature for 30 minutes.
13. Stop reaction by adding 100 μL of Stop Solution to each well.
14. Measure absorbance on a plate reader at 450 nm.

Calcul des résultats:

Duplicate absorbance values should be within 10% of each other. Care should be taken when interpreting data with differences in absorbance values greater than 10%.

1. Prepare a standard curve to determine the amount of Hsp90α in an unknown sample. Plot the average absorbance obtained for each standard concentration on the vertical (Y) axis versus the corresponding Hsp90α concentration on the horizontal (X) axis using graph paper or curve-fitting software.

2. Calculate the Hsp90α concentration in unknown samples using the prepared standard curve. Determine the amount of Hsp90α in each unknown sample by noting the Hsp90α concentration (X axis) that correlates with the absorbance value (Y axis) obtained for the unknown sample.

3. Multiply the Hsp90α concentration obtained by the dilution factor to determine the amount of Hsp90α in the undiluted sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour HSP90 Kit ELISA (ABIN2964826)

Miyazaki, Nakamura, Hineno, Kobayashi, Kinoshita, Yoshida, Ikeda: "Elevation of serum heat-shock protein levels in amyotrophic lateral sclerosis." dans: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 37, Issue 8, pp. 1277-81, (2016) (PubMed).

Tian, He, Fu, Lin, Tang, Huang, Guo, Sun: "High expression of heat shock protein 90 alpha and its significance in human acute leukemia cells." dans: Gene, Vol. 542, Issue 2, pp. 122-8, (2014) (PubMed).

Détail du antigène

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Autre désignation: HSP90 (HSP90 Produits)

Synonymes: EL52 Kit ELISA, HSP86 Kit ELISA, HSP89A Kit ELISA, HSP90A Kit ELISA, HSP90N Kit ELISA, HSPC1 Kit ELISA, HSPCA Kit ELISA, HSPCAL1 Kit ELISA, HSPCAL4 Kit ELISA, HSPN Kit ELISA, Hsp89 Kit ELISA, Hsp90 Kit ELISA, LAP2 Kit ELISA, git10 Kit ELISA, swo1 Kit ELISA, HSP90 Kit ELISA, htpG Kit ELISA, SCBAC25F8.08 Kit ELISA, 23.m06066 Kit ELISA, 17.m07646 Kit ELISA, HSP90-1 Kit ELISA, 143198_at Kit ELISA, 83 Kit ELISA, 83K HSP Kit ELISA, DMHSP82 Kit ELISA, E(sev)3A Kit ELISA, E(sina)2 Kit ELISA, HSP82 Kit ELISA, HSP83 Kit ELISA, ORF1 Kit ELISA, Su(Raf)3A Kit ELISA, anon-EST:Liang-2.53 Kit ELISA, anon-WO0068693 Kit ELISA, anon-WO0140519.209 Kit ELISA, clone 2.53 Kit ELISA, en(lz)3C/4C Kit ELISA, hsp84 Kit ELISA, l(3)j5C2 Kit ELISA, ms(3)08445 Kit ELISA, stc Kit ELISA, DmelCG1242 Kit ELISA, CG1242 Kit ELISA, Hsp86 Kit ELISA, Hspca Kit ELISA, 86kDa Kit ELISA, 89kDa Kit ELISA, AL024080 Kit ELISA, AL024147 Kit ELISA, Hsp86-1 Kit ELISA, hsp4 Kit ELISA, hsp86 Kit ELISA, hsp89 Kit ELISA, hsp90 Kit ELISA, hsp90a Kit ELISA, hspc1 Kit ELISA, hspca Kit ELISA, hspn Kit ELISA, lap2 Kit ELISA, Hsp90alpha Kit ELISA, heat shock protein 90 alpha family class A member 1 Kit ELISA, heat shock protein Hsp90 Kit ELISA, Hsp90 chaperone Kit ELISA, Heat shock protein 90 Kit ELISA, heat shock protein 90 Kit ELISA, chaperone protein HtpG Kit ELISA, molecular chaperone HtpG Kit ELISA, Heat Shock Protein 90 Kit ELISA, uncharacterized LOC100384473 Kit ELISA, Heat shock protein 83 Kit ELISA, heat shock protein 90, alpha (cytosolic), class A member 1 Kit ELISA, heat shock protein 90 alpha family class B member 1 Kit ELISA, heat shock protein 90kDa alpha family class A member 1 L homeolog Kit ELISA, heat shock protein HSP 90-alpha Kit ELISA, HSP90AA1 Kit ELISA, hsp90 Kit ELISA, HSP90 Kit ELISA, daf-21 Kit ELISA, htpG Kit ELISA, SCO7516 Kit ELISA, MAP_RS10510 Kit ELISA, TP04_0646 Kit ELISA, TP01_0934 Kit ELISA, APH_RS03525 Kit ELISA, GbCGDNIH1_0315 Kit ELISA, MAV_2118 Kit ELISA, HSP90C Kit ELISA, BBOV_IV008400 Kit ELISA, BBOV_III007380 Kit ELISA, ACICU_00312 Kit ELISA, ECL_01244 Kit ELISA, YE105_C1172 Kit ELISA, pco153543(105) Kit ELISA, Hsp83 Kit ELISA, Hsp90aa1 Kit ELISA, HSP90AB1 Kit ELISA, hsp90aa1.1.L Kit ELISA, LOC108698781 Kit ELISA

Sujet: HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90- regulated proteins that have been discovered to date are involved in cell signaling. The number of proteins now known to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(3). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function.

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals