FGF2 Kit ELISA

N° du produit ABIN3198428

Kit ELISA FGF2 Humain, Colorimetric test pour la quantification de Humain FGF2.

Aperçu rapide pour FGF2 Kit ELISA (ABIN3198428)

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.63 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit FGF2 Kit ELISA

Seuil minimal de détection: 15.63 pg/mL

Fonction: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytical Method: Quantitative

Specificité: FGF basic ELISA Kit, Human

Ingrédients:

• Plate
• Standard
• Diluent

Matériel non inclus: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: protect your eyes

Stock: 4 °C

Stockage commentaire: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Détail du antigène

Antigène: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Autre désignation: FGF Basic

Sujet: Basic fibroblast growth factor, also known as bFGF, FGF2 or FGF-β, is a member of the fibroblast growth factor family. During both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF to mediate angiogenesis.

ID gène: 2246

NCBI Accession: NM_000800

UniProt: P05230

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins