TNFSF8 Kit ELISA

N° du produit ABIN411376

Détail du produit TNFSF8 Kit ELISA

Antigène: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Épitope: AA 68-239

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD30L

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q68-D239

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <6pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q68-D239

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse CD30L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30L has been precoated onto 96-well plates. Standards(NSO, Q68-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30L amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CD30L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CD30L standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 92, Standard deviation: 4.51, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 349, Standard deviation: 24.08, CV(%): 6.9
• Sample 3: n=16, Mean(pg/ml): 661, Standard deviation: 40.98, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 6.93, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 357, Standard deviation: 25.35, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 711, Standard deviation: 51.90, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Autre désignation: TNFSF8 (TNFSF8 Produits)

Synonymes: CD153 Kit ELISA, CD30LG Kit ELISA, Cd30l Kit ELISA, CD30L Kit ELISA, TNFRSF8 Kit ELISA, TNFSF8 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 8 Kit ELISA, TNF superfamily member 8 Kit ELISA, tumor necrosis factor superfamily member 8 Kit ELISA, Tnfsf8 Kit ELISA, TNFSF8 Kit ELISA

Sujet:

Protein Function: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Background: The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

Synonyms: Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,CD153,Tnfsf8,Cd30l, Cd30lg,

Full Gene Name: Tumor necrosis factor ligand superfamily member 8

Cellular Localisation: Membrane, Single-pass type II membrane protein.

ID gène: 21949

UniProt: P32972