Anti-PAR2 Antibody (IgG) Kit ELISA

N° du produit ABIN454019

Détail du produit

Antigène: Anti-PAR2 Antibody (IgG)

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Fonction: The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.

Analytical Method: Quantitative

Attributs du produit: The anti-Thrombin-Receptor-like-1-Antibody-EIA is an antibody screening test.

Information d'application

Plaque: Pre-coated

Protocole: The Thrombin-Receptor-like-1 has been pre-coated onto a microtiter plate. During the first incubation the anti-Thrombin-Receptor-like-1-Antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Thrombin-Receptor-like-1-Antibody.

Procédure de l'essai:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Précision du teste: Intraassay-Precision:7,83% Interassay-Precision:9,12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Anti-PAR2 Antibody (IgG)

Classe de substances: Antibody

Sujet: The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP.