KIT Ligand Kit ELISA

N° du produit ABIN4881551

Détail du produit KIT Ligand Kit ELISA

Antigène: KIT Ligand (KITLG)

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Canine SCF/KITLG ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Canine SCF.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: SCF (KITLG (KITLG Produits)

Synonymes: Xkl-1 Kit ELISA, Xsl Kit ELISA, Xsl-1 Kit ELISA, Xsl-2 Kit ELISA, steel Kit ELISA, KITLG Kit ELISA, SCF Kit ELISA, kitl Kit ELISA, kl-1 Kit ELISA, mgf Kit ELISA, scf Kit ELISA, FPH2 Kit ELISA, KL-1 Kit ELISA, Kitl Kit ELISA, MGF Kit ELISA, SF Kit ELISA, SHEP7 Kit ELISA, Clo Kit ELISA, Con Kit ELISA, Gb Kit ELISA, Kitlg Kit ELISA, Mgf Kit ELISA, SLF Kit ELISA, Sl Kit ELISA, blz Kit ELISA, contrasted Kit ELISA, 710-712 Kit ELISA, CSF Kit ELISA, KITL Kit ELISA, KIT ligand L homeolog Kit ELISA, KIT ligand Kit ELISA, kit ligand Kit ELISA, kitlg.L Kit ELISA, KITLG Kit ELISA, kitlg Kit ELISA, Kitl Kit ELISA, Kitlg Kit ELISA

ID gène: 403507

UniProt: Q06220

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway