Aggrecan Kit ELISA
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- Antigène Voir toutes Aggrecan (ACAN) Kits ELISA
- Aggrecan (ACAN)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 41-10.000 pg/mL
- Seuil minimal de détection
- 41 pg/mL
- Application
- ELISA
- Fonction
- Human Aggrecan ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human Aggrecan.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Aggrecan (ACAN) Kits ELISA
- Aggrecan (ACAN)
- Autre désignation
- Aggrecan (ACAN Produits)
- Synonymes
- AGC1 Kit ELISA, AGCAN Kit ELISA, CSPG1 Kit ELISA, CSPGCP Kit ELISA, MSK16 Kit ELISA, SEDK Kit ELISA, Agc Kit ELISA, Agc1 Kit ELISA, Cspg1 Kit ELISA, b2b183Clo Kit ELISA, cmd Kit ELISA, aggrecan Kit ELISA, aggrecan core protein Kit ELISA, ACAN Kit ELISA, Acan Kit ELISA, LOC100380783 Kit ELISA, AGC Kit ELISA
- UniProt
- P16112
- Pathways
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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