AKR1C3 Kit ELISA

N° du produit ABIN4881786

Détail du produit AKR1C3 Kit ELISA

Antigène: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human AKR1C3 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human BIRC4.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Autre désignation: AKR1C3 (AKR1C3 Produits)

Synonymes: AKR1C3 Kit ELISA, PGFS Kit ELISA, MGC134333 Kit ELISA, DD3 Kit ELISA, DDX Kit ELISA, HA1753 Kit ELISA, HAKRB Kit ELISA, HAKRe Kit ELISA, HSD17B5 Kit ELISA, hluPGFS Kit ELISA, Akr1c18 Kit ELISA, aldo-keto reductase family 1, member C3 L homeolog Kit ELISA, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II) Kit ELISA, aldo-keto reductase family 1 member C2 Kit ELISA, aldo-keto reductase family 1 member C3 Kit ELISA, aldo-keto reductase family 1, member C3 Kit ELISA, akr1c3.L Kit ELISA, AKR1C3 Kit ELISA, LOC722496 Kit ELISA, Akr1c3 Kit ELISA

ID gène: 8644

UniProt: P42330

Pathways: Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus