COL1A1 Kit ELISA

N° du produit ABIN4882440

Détail du produit COL1A1 Kit ELISA

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Collagen alpha-1 chain ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human LRRN1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Autre désignation: Collagen alpha-1 Chain (COL1A1 Produits)

Synonymes: OI4 Kit ELISA, Col1a-1 Kit ELISA, Cola-1 Kit ELISA, Cola1 Kit ELISA, Mov-13 Kit ELISA, Mov13 Kit ELISA, COLIA1 Kit ELISA, col1a1 Kit ELISA, MGC52532 Kit ELISA, COL1A1 Kit ELISA, COL1A2 Kit ELISA, alpha1(I) Kit ELISA, cb21 Kit ELISA, sb:cb21 Kit ELISA, sb:cb384 Kit ELISA, wu:fa99c12 Kit ELISA, wu:fb02c06 Kit ELISA, alpha3(I) Kit ELISA, col1a3 Kit ELISA, hm:zeh0348 Kit ELISA, wu:fa95h05 Kit ELISA, wu:fd02a10 Kit ELISA, wu:fj59a10 Kit ELISA, collagen type I alpha 1 chain Kit ELISA, collagen, type I, alpha 1 Kit ELISA, collagen, type I, alpha 1 S homeolog Kit ELISA, collagen alpha-1(I) chain Kit ELISA, collagen type I alpha 2 chain Kit ELISA, collagen, type I, alpha 1a Kit ELISA, collagen 1a1 Kit ELISA, collagen, type I, alpha 1b Kit ELISA, COL1A1 Kit ELISA, Col1a1 Kit ELISA, col1a1.S Kit ELISA, LOC397571 Kit ELISA, COL1A2 Kit ELISA, col1a1a Kit ELISA, col1a1 Kit ELISA, col1a1b Kit ELISA

ID gène: 1277

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding