COMT Kit ELISA

N° du produit ABIN4882446

Détail du produit COMT Kit ELISA

Antigène: COMT (Catechol-O-Methyltransferase (COMT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human COMT ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human LTA4H.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: COMT (Catechol-O-Methyltransferase (COMT))

Autre désignation: COMT (COMT Produits)

Synonymes: Comt1 Kit ELISA, D16Wsu103e Kit ELISA, D330014B15Rik Kit ELISA, COMT Kit ELISA, mb-comt Kit ELISA, s-comt Kit ELISA, comt Kit ELISA, MGC154444 Kit ELISA, zgc:114157 Kit ELISA, si:dkey-13a21.15 Kit ELISA, zK13A21.15 Kit ELISA, zgc:162236 Kit ELISA, catechol-O-methyltransferase Kit ELISA, microRNA 4761 Kit ELISA, catechol-O-methyltransferase S homeolog Kit ELISA, methyltransferase Kit ELISA, catechol-o-methyltransferase Kit ELISA, catechol-O-methyltransferase a Kit ELISA, catechol-O-methyltransferase b Kit ELISA, catechol O-methyltransferase Kit ELISA, COMT Kit ELISA, Comt Kit ELISA, MIR4761 Kit ELISA, comt Kit ELISA, comt.2 Kit ELISA, comt.S Kit ELISA, Rv1703c Kit ELISA, Mb1729c Kit ELISA, comta Kit ELISA, comtb Kit ELISA, LOC100354142 Kit ELISA

ID gène: 1312

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome