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EPH Receptor B2 Kit ELISA

EPHB2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882751
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    EPH Receptor B2 (EPHB2)
    Reactivité
    • 4
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human EphB2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Tenascin X.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes EPH Receptor B2 (EPHB2) Kits ELISA
    EPH Receptor B2 (EPHB2)
    Autre désignation
    EphB2 (EPHB2 Produits)
    Synonymes
    CAPB Kit ELISA, DRT Kit ELISA, EK5 Kit ELISA, EPHT3 Kit ELISA, ERK Kit ELISA, Hek5 Kit ELISA, PCBC Kit ELISA, Tyro5 Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, RGD1564232 Kit ELISA, Cek5 Kit ELISA, Drt Kit ELISA, ETECK Kit ELISA, Erk Kit ELISA, Nuk Kit ELISA, Prkm5 Kit ELISA, Qek5 Kit ELISA, Sek3 Kit ELISA, cek5 Kit ELISA, ephb2 Kit ELISA, xEphB2 Kit ELISA, si:ch73-160e9.1 Kit ELISA, tpx2 Kit ELISA, wu:fb09e10 Kit ELISA, wu:fc64a03 Kit ELISA, wu:fi18g12 Kit ELISA, zgc:112296 Kit ELISA, EPH receptor B2 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, Eph receptor B2 Kit ELISA, EPH receptor B2 L homeolog Kit ELISA, eph receptor B2a Kit ELISA, eph receptor B2b Kit ELISA, EPHB2 Kit ELISA, MAPK1 Kit ELISA, Ephb2 Kit ELISA, ephb2.L Kit ELISA, ephb2a Kit ELISA, ephb2b Kit ELISA
    ID gène
    2048
    UniProt
    P29323
    Pathways
    Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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