EPH Receptor B2 Kit ELISA

N° du produit ABIN6730732

Détail du produit EPH Receptor B2 Kit ELISA

Antigène: EPH Receptor B2 (EPHB2)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse EphB2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse EphB2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: EphB2 (EPHB2 Produits)

Synonymes: CAPB Kit ELISA, DRT Kit ELISA, EK5 Kit ELISA, EPHT3 Kit ELISA, ERK Kit ELISA, Hek5 Kit ELISA, PCBC Kit ELISA, Tyro5 Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, RGD1564232 Kit ELISA, Cek5 Kit ELISA, Drt Kit ELISA, ETECK Kit ELISA, Erk Kit ELISA, Nuk Kit ELISA, Prkm5 Kit ELISA, Qek5 Kit ELISA, Sek3 Kit ELISA, cek5 Kit ELISA, ephb2 Kit ELISA, xEphB2 Kit ELISA, si:ch73-160e9.1 Kit ELISA, tpx2 Kit ELISA, wu:fb09e10 Kit ELISA, wu:fc64a03 Kit ELISA, wu:fi18g12 Kit ELISA, zgc:112296 Kit ELISA, EPH receptor B2 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, Eph receptor B2 Kit ELISA, EPH receptor B2 L homeolog Kit ELISA, eph receptor B2a Kit ELISA, eph receptor B2b Kit ELISA, EPHB2 Kit ELISA, MAPK1 Kit ELISA, Ephb2 Kit ELISA, ephb2.L Kit ELISA, ephb2a Kit ELISA, ephb2b Kit ELISA

UniProt: P54763

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins