FCGR3B Kit ELISA

N° du produit ABIN4882817

Détail du produit FCGR3B Kit ELISA

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Fc gamma RIIIB (CD16b) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Autre désignation: Fc gamma RIIIB (FCGR3B Produits)

Synonymes: CD16 Kit ELISA, CD16b Kit ELISA, FCG3 Kit ELISA, FCGR3 Kit ELISA, FCR-10 Kit ELISA, FCRIII Kit ELISA, FCRIIIb Kit ELISA, FcRIII Kit ELISA, Fc fragment of IgG receptor IIIb Kit ELISA, Fc receptor, IgG, low affinity III Kit ELISA, Fc fragment of IgG receptor IIIa Kit ELISA, Fc-gamma RIII Kit ELISA, FCGR3B Kit ELISA, Fcgr3 Kit ELISA, FCGR3A Kit ELISA, FCGR3 Kit ELISA

ID gène: 2215

UniProt: O75015