ST14 Kit ELISA

N° du produit ABIN4883709

Détail du produit ST14 Kit ELISA

Antigène: ST14 (Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Matriptase/ST14 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Matriptase.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ST14 (Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))

Autre désignation: Matriptase (ST14 Produits)

Synonymes: HAI Kit ELISA, MT-SP1 Kit ELISA, MTSP1 Kit ELISA, PRSS14 Kit ELISA, SNC19 Kit ELISA, TADG15 Kit ELISA, TMPRSS14 Kit ELISA, XMT-SP1 Kit ELISA, hai Kit ELISA, mt-sp1 Kit ELISA, mtsp1 Kit ELISA, prss14 Kit ELISA, snc19 Kit ELISA, st14 Kit ELISA, st14a Kit ELISA, tadg15 Kit ELISA, tmprss1 Kit ELISA, Epithin Kit ELISA, Prss14 Kit ELISA, Tmprss14 Kit ELISA, mCAP3 Kit ELISA, matriptase Kit ELISA, suppression of tumorigenicity 14 Kit ELISA, suppression of tumorigenicity 14 L homeolog Kit ELISA, suppression of tumorigenicity 14 (colon carcinoma) Kit ELISA, ST14 Kit ELISA, st14.L Kit ELISA, St14 Kit ELISA

ID gène: 6768

UniProt: Q9Y5Y6