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Paxillin Kit ELISA

PXN Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884307
  • Antigène Voir toutes Paxillin (PXN) Kits ELISA
    Paxillin (PXN)
    Reactivité
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Paxillin ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Paxillin (PXN) Kits ELISA
    Paxillin (PXN)
    Autre désignation
    Paxillin (PXN Produits)
    Synonymes
    AW108311 Kit ELISA, AW123232 Kit ELISA, Pax Kit ELISA, CG18061 Kit ELISA, CG18576 Kit ELISA, CG31794 Kit ELISA, CT40481 Kit ELISA, CT42454 Kit ELISA, DPaxillin Kit ELISA, DPxn Kit ELISA, DPxn37 Kit ELISA, Dmel\\CG31794 Kit ELISA, Dpax Kit ELISA, DpaxA Kit ELISA, PDLP Kit ELISA, dPax Kit ELISA, pax Kit ELISA, wu:fw71f12 Kit ELISA, PXN Kit ELISA, LOC100220858 Kit ELISA, paxillin Kit ELISA, Paxillin Kit ELISA, paxillin S homeolog Kit ELISA, paxillin a Kit ELISA, PXN Kit ELISA, Pxn Kit ELISA, Pax Kit ELISA, pxn.S Kit ELISA, pxna Kit ELISA, LOC696336 Kit ELISA, pxn Kit ELISA
    ID gène
    5829
    UniProt
    P49023
    Pathways
    Signalisation MAPK, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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