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RYK Kit ELISA

RYK Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884386
  • Antigène Voir toutes RYK Kits ELISA
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Ryk ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes human Ryk. Other species not yet determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product RYK Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes RYK Kits ELISA
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Autre désignation
    Ryk (RYK Produits)
    Synonymes
    RYK Kit ELISA, jtk5 Kit ELISA, ryk1 Kit ELISA, jtk5a Kit ELISA, d3s3195 Kit ELISA, wu:fb37b12 Kit ELISA, wu:fi05b06 Kit ELISA, zgc:158381 Kit ELISA, MGC114645 Kit ELISA, D3S3195 Kit ELISA, JTK5 Kit ELISA, JTK5A Kit ELISA, RYK1 Kit ELISA, AW536699 Kit ELISA, ERK-3 Kit ELISA, Vik Kit ELISA, receptor-like tyrosine kinase Kit ELISA, receptor-like tyrosine kinase S homeolog Kit ELISA, tyrosine-protein kinase RYK Kit ELISA, RYK Kit ELISA, ryk Kit ELISA, ryk.S Kit ELISA, LOC100541106 Kit ELISA, Ryk Kit ELISA
    ID gène
    6259
    UniProt
    P34925
    Pathways
    Signalisation RTK, Signalisation WNT, Regulation of Cell Size
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