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RYK Kit ELISA

RYK Reactivité: Humain pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886279
  • Antigène Voir toutes RYK Kits ELISA
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Épitope
    pTyr
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Phosphotyrosine RYK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine RYK in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-RYK.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes RYK Kits ELISA
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Autre désignation
    RYK (RYK Produits)
    Synonymes
    RYK Kit ELISA, jtk5 Kit ELISA, ryk1 Kit ELISA, jtk5a Kit ELISA, d3s3195 Kit ELISA, wu:fb37b12 Kit ELISA, wu:fi05b06 Kit ELISA, zgc:158381 Kit ELISA, MGC114645 Kit ELISA, D3S3195 Kit ELISA, JTK5 Kit ELISA, JTK5A Kit ELISA, RYK1 Kit ELISA, AW536699 Kit ELISA, ERK-3 Kit ELISA, Vik Kit ELISA, receptor-like tyrosine kinase Kit ELISA, receptor-like tyrosine kinase S homeolog Kit ELISA, tyrosine-protein kinase RYK Kit ELISA, RYK Kit ELISA, ryk Kit ELISA, ryk.S Kit ELISA, LOC100541106 Kit ELISA, Ryk Kit ELISA
    ID gène
    6259
    UniProt
    P34925
    Pathways
    Signalisation RTK, Signalisation WNT, Regulation of Cell Size
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